Plasmid

Part:BBa_I713023:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pKD-pBL-lambda


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2198
    Illegal XbaI site found at 2602
    Illegal XbaI site found at 3406
    Illegal SpeI site found at 3529
    Illegal PstI site found at 2537
    Illegal PstI site found at 3384
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3047
    Illegal SpeI site found at 3529
    Illegal PstI site found at 2537
    Illegal PstI site found at 3384
    Illegal NotI site found at 2514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3372
    Illegal BamHI site found at 3535
    Illegal XhoI site found at 2157
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2198
    Illegal XbaI site found at 2602
    Illegal XbaI site found at 3406
    Illegal SpeI site found at 3529
    Illegal PstI site found at 2537
    Illegal PstI site found at 3384
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2198
    Illegal XbaI site found at 2602
    Illegal XbaI site found at 3406
    Illegal SpeI site found at 3529
    Illegal PstI site found at 2537
    Illegal PstI site found at 3384
    Illegal NgoMIV site found at 2404
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1291
    Illegal BsaI.rc site found at 2936
    Illegal SapI site found at 208
    Illegal SapI site found at 2344


Design Notes

By inserting the KpnI-XhoI fragment from BBa_I713025 into BBa_I713021(KpnI-SalI)


Source

BBa_I713021 BBa_I713025

References